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Cytomatrix |
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Long-Term Three Dimensional Hematopoietic Stem Cell Culture Abstract |
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J. Bagley1, N. Banu1, M. Rosenzweig2, and M. Pykett1.
1Cytomatrix, 100 Inman St., Cambridge, MA 02139, 2New England Regional Primate Research Center, Harvard Medical School, Boston, MA |
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The culture of hematopoietic progenitor cells (HPCs) in three-dimensional matrices imparts advantages to the survival of these cells ex vivo. We have used a novel, highly porous, three-dimensional matrix termed CellfoamTM to support HPC survival and mulitpotency in long-term-cultures to which no exogenous cytokines have been added. The survival, phenotype and multipotency of HPC’s cultured for up to several months in CellfoamTM was compared to control systems including fibronectin coated plastic, bone marrow stroma co-cultures and other three dimensional materials. CellfoamTM was found to support enhanced HPC survival, colony forming activity and the elaboration of long-term culture initiating cells relative to alternative systems. These finding point to new approaches which may improve the in vitro preservation of stem cells and which may have implications in clinical areas such as stem cell expansion, bone marrow transplantation and gene therapy.   |
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